Data di Pubblicazione:
2013
Citazione:
Factors affecting T cell responses induced by fully synthetic glyco-gold-nanoparticles / S. Fallarini, T. Paoletti, C. Orsi Battaglini, P. Ronchi, L. Lay, R. Bonomi, S. Jha, F. Mancin, P. Scrimin, G. Lombardi. - In: NANOSCALE. - ISSN 2040-3364. - 5:1(2013), pp. 390-400.
Abstract:
We have synthesized and characterized nearly monodisperse and highly pure gold nanoparticles (2 and
5 nm) coated with non-immunoactive mono- and disaccharides, modelled after the capsular
polysaccharide of serogroup A of the Neisseria meningitidis bacterium. We have used them to test their
ability to induce immune cell responses as a consequence of their multivalency. The results indicate that
they are indeed immunoactive and that immunoactivity is strongly dependent on size, and larger, 5 nm
nanoparticles perform far better than smaller, 2 nm ones. Immune response (activation of macrophages)
initiates with the whole nanoparticle recognition by the surface of antigen-presenting cells, independent
of the saccharide oligomerization (or charge) on the nanoparticle surface. The induction of T cell
proliferation and the increase of IL-2 levels, a consequence of the expression of MHC II involved in antigen
presentation, require the presence of a disaccharide on the nanoparticle, not just a monosaccharide. A
possible explanation is that, at this stage, the saccharides are detached from the gold surface. These
results may provide leads for designing new saccharide-based, nanoparticle-conjugate vaccines.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
S. Fallarini, T. Paoletti, C. Orsi Battaglini, P. Ronchi, L. Lay, R. Bonomi, S. Jha, F. Mancin, P. Scrimin, G. Lombardi
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