Data di Pubblicazione:
2000
Citazione:
Polymorphism of microsatellite loci and parentage identification in some Italian dog breeds / M. Polli, S.P. Marelli, M. Zanotti, L. Guidobono Cavalchini. ((Intervento presentato al 27. convegno International Conference on Animal Genetics tenutosi a Saint Paul, Minnesota nel 2000.
Abstract:
Canine microsatellites are powerful tools for parentage verification to increase the value of pedigrees and
to give selective criteria in dog breeding. The ENCI (Ente Nazionale della Cinofilia Italiana) only recently
has started to test dogs in our laboratory to resolve doubtful breeding cases; the interest in a correct
litter's registration in stud books is growing up. In the present study we investigated the variability of ten
canine microsatellites loci (AHT121, CXX20-123-263-403-2159-2137-2138-2001-2132) in ten Italian pure
breeds (Bergamasco, Bolognese, Bracco Italiano, Cirneco dell'Etna, Corso, Fonnese, Lagotto, Maremma
sheepdog, Segugio Italiano, Spinone Italiano, Volpino Italiano). All the samples have been collected from
animals unrelated in two generations. DNA extracted from blood or buccal swabs was PCR amplified and
labeled fragments were electrophoresed on ABI 377 automated sequencer. The differences in the
observed allele frequencies between the breeds and the Polymorphism Information Content were
examined and evaluated for each population and for each locus. We investigated also the most
polymorphic microsatellite markers for parentage control and we calculated the cumulative exclusion
probabilities for all the breeds and for all the analysed loci. In addition the obtained allele frequencies
were used to estimate the genetic distances for all the considered breeds. Analysis of exclusion power
(PE), ranging from 98,50% and 99,93%, using the most informative markers, demonstrates, the
microsatellite efficiency and their potential use in stud book registrations of Italian pure breeds.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
M. Polli, S.P. Marelli, M. Zanotti, L. Guidobono Cavalchini
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