Ionizing radiations increase the activity of the cellsurface glycohydrolases and the plasma membrane ceramide content
Articolo
Data di Pubblicazione:
2012
Citazione:
Ionizing radiations increase the activity of the cellsurface glycohydrolases and the plasma membrane ceramide content / M. Aureli, R. Bassi, A. Prinetti, E. Chiricozzi, B. Pappalardi, V. Chigorno, N. Di Muzio, N. Loberto, S. Sonnino. - In: GLYCOCONJUGATE JOURNAL. - ISSN 0282-0080. - 2012:29(2012), pp. 585-597. [10.1007/s10719-012-9385-2]
Abstract:
Abstract We detected significant levels of β-glucosidase,
β-galactosidase, sialidase Neu3 and sphingomyelinase activities
associated with the plasma membrane of fibroblasts
from normal and Niemann-Pick subjects and of cells from
breast, ovary, colon and neuroblastoma tumors in culture.
All of the cells subjected to ionizing radiations showed an
increase of the activity of plasma membrane β-glucosidase,
β-galactosidase and sialidase Neu3, in addition of the well
known increase of activity of plasma membrane sphingomyelinase,
under similar conditions. Human breast cancer
cell line T47D was studied in detail. In these cells the
increase of activity of β-glucosidase and β-galactosidase
was parallel to the increase of irradiation dose up to 60 Gy
and continued with time, at least up to 72 h from irradiation.
β-glucosidase increased up to 17 times and β-galactosidase
up to 40 times with respect to control. Sialidase Neu3 and
sphingomyelinase increased about 2 times at a dose of
20 Gy but no further significant differences were observed
with increase of radiation dose and time. After irradiation,
we observed a reduction of cell proliferation, an increase of
apoptotic cell death and an increase of plasma membrane
ceramide up to 3 times, with respect to control cells. Tritiated
GM3 ganglioside has been administered to T47D cells
under conditions that prevented the lysosomal catabolism.
GM3 became component of the plasma membranes and was
transformed into LacCer, GlcCer and ceramide. The quantity
of ceramide produced in irradiated cells was about two
times that of control cells.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Apoptosis; Ceramide; Glycosidases; Plasma membrane; Radiations; Radiotherapy
Elenco autori:
M. Aureli, R. Bassi, A. Prinetti, E. Chiricozzi, B. Pappalardi, V. Chigorno, N. Di Muzio, N. Loberto, S. Sonnino
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