6-Keto-prostaglandin E1-sensitive adenylate cyclase and binding sites in membranes from platelets and cultured smooth muscle cells
Articolo
Data di Pubblicazione:
1984
Citazione:
6-Keto-prostaglandin E1-sensitive adenylate cyclase and binding sites in membranes from platelets and cultured smooth muscle cells / D. Oliva, F. Bernini, A. Corsini, S. Nicosia. - In: BIOCHEMICAL PHARMACOLOGY. - ISSN 0006-2952. - 33:23(1984 Dec 01), pp. 3755-3758. [10.1016/0006-2952(84)90036-4]
Abstract:
6-keto-PGE1 elicits the same biological effects as PGI2 in human platelets and in rabbit aorta and mesenteric artery, being, however, less potent. We report here that 6-keto-PGE1 dose-dependently stimulates adenylate cyclase activity in membranes of human platelets and cultured myocytes from rabbit aorta and mesenteric artery. The extent of stimulation of the enzyme by 6-keto-PGE1 is the same as elicited by PGI2, while the apparent affinity is lower than that of prostacyclin, both in platelets and in vascular smooth muscle cells. At the level of platelet membranes, 6-keto-PGE1 interacts with the binding sites labelled by PGI2. However, in platelets as well as in mesenteric artery myocytes, 6-keto-PGE1 interacts with only one class of sites as demonstrated either by binding or by adenylate cyclase studies, whereas PGI2 in the same conditions recognizes two different classes.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Alprostadil ; Animals ; Vasodilator Agents ; Mesenteric Arteries ; Aorta ; Humans ; Rabbits ; Adenylate Cyclase ; Binding Sites ; Blood Platelets ; Muscle, Smooth, Vascular ; Cells, Cultured ; Kinetics ; Cell Membrane ; Epoprostenol ; Prostaglandins E ; Male
Elenco autori:
D. Oliva, F. Bernini, A. Corsini, S. Nicosia
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