Data di Pubblicazione:
2012
Citazione:
Influence of recovery methods and extenders on bull epididymal spermatozoa quality / F. Turri, M. Madeddu, T.M. Gliozzi, G. Gandini, F. Pizzi. - In: REPRODUCTION IN DOMESTIC ANIMALS. - ISSN 0936-6768. - 47:5(2012 Oct), pp. 712-717. [10.1111/j.1439-0531.2011.01948.x]
Abstract:
The aim of this study was to compare the effects of two
extraction methods in combination with two different extenders
in bull epididymal sperm collection. Testes from 23 sexually
mature Limousine bulls were collected at the abattoir.
Epididymal sperm recovery was performed using both the
float-up (FL) and the retrograde flushing (RF) technique.
Within extraction methods, half testes were processed with a
Tris egg yolk extender and half with a Tris egg yolk-free
extender. Sperm concentration, motility, viability and morphology
were evaluated. Sperm concentration was not significantly
different between methods. Flushing technique was
significantly better than the FL method in terms of sperm
quality, considering total motility (80.3 ± 2.3% vs 71.6 ±
2.0%, p < 0.001, respectively) and viability (84.5 ± 1.5% vs
77.2 ± 1.3%, p < 0.001, respectively). Egg yolk influenced
positively motility and morphology in the FL method, whereas
decreased viability in flushed samples. Results suggest the use
of the RF technique to collect cattle epididymal sperm.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
egg-yolk ; bull ; epididymal sperm ; sperm collection ; cryopreservation ; motility, viability ; morphology
Elenco autori:
F. Turri, M. Madeddu, T.M. Gliozzi, G. Gandini, F. Pizzi
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