Data di Pubblicazione:
2001
Citazione:
Functional genomics of Arabidopsis photosynthesis / P. Pesaresi, C. Varotto, E. Richly, J. Kurth, F. Salamini, D. Leister. - In: PLANT PHYSIOLOGY AND BIOCHEMISTRY. - ISSN 0981-9428. - 39:3-4(2001), pp. 285-294.
Abstract:
The study of photosynthesis in higher plants will benefit significantly from the sequencing of the entire genome of
Arabidopsis thaliana and from the recent development of new genomic technologies. For large sets of genes, it is now possible
to identify their functions and levels of expression, and the post-translational modifications and interactions of their products.
This functional genomic approach to photosynthesis is summarised here by considering the application of knock-out mutant
screens (forward genetics), the identification of mutant alleles of genes whose sequence is known (reverse genetics), the use of
microarrays to detect gene expression at genome level, the separation of proteins by two-dimensional electrophoresis, and their
identification by mass spectrometry and bioinformatics. This emerging, integrated approach to the elucidation of gene function
is presented and its potential is discussed.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Arabidopsis; Functional genomics; Mutant; Photosynthesis proteomics; Reverse genetics; Transcriptomics
Elenco autori:
P. Pesaresi, C. Varotto, E. Richly, J. Kurth, F. Salamini, D. Leister
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