The human Evi-1 gene is located on chromosome 3q24-q28 but is not rearranged in three cases of acute nonlymphocytic leukemias containing t(3;5)(q25;q34) translocations
Articolo
Data di Pubblicazione:
1990
Citazione:
The human Evi-1 gene is located on chromosome 3q24-q28 but is not rearranged in three cases of acute nonlymphocytic leukemias containing t(3;5)(q25;q34) translocations / K. Morishita, E. Parganas, C. Bartholomew, N. Sacchi, M.B. Valentine, S.C. Raimondi, M.M. Le Beau, J.N. Ihle. - In: ONCOLOGY RESEARCH. - ISSN 0965-0407. - 5:3(1990), pp. 221-231.
Abstract:
The murine Evi-1 gene encodes a protein that has multiple 28-amino acid repeats containing the consensus sequence found in the zinc finger domains of many transcriptional regulatory proteins. Activation of the expression of the Evi-1 gene is frequently found in murine myeloid leukemias and leukemia cell lines and is due to retroviral insertions in the 5' region of the gene in either the Evi-1 or the CB-1/FIM3 common sites of viral integrations. To examine the role of the Evi-1 gene in human leukemias we have cloned regions of the human locus corresponding to the coding region of the gene and regions corresponding to the Evi-1 and CB-1/FIM3 common sites of integrations. Using these probes we demonstrate that the human Evi-1 gene maps to chromosome 3q24-q28 in a region that is translocated in acute nonlymphocytic leukemias with a t(3;5)(q25;q34). By in situ hybridization with metaphase chromosomes from one patient with a 3;5 translocation, the Evi-1 gene was found to be translocated to the derivative 5 chromosome. However, no rearrangements were detected by Southern blot analysis with DNAs from three patients with a t(3;5) using probes from the Evi-1 or CB-1/FIM3 loci. No Evi-1 transcripts were detected with RNA from leukemic blasts of one patient with a t(3;5).
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Acute nonlymphocytic leukemia; Human chromosome 3; Human Evi-1; Myeloid transformation
Elenco autori:
K. Morishita, E. Parganas, C. Bartholomew, N. Sacchi, M.B. Valentine, S.C. Raimondi, M.M. Le Beau, J.N. Ihle
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