Data di Pubblicazione:
2001
Citazione:
Preparation of RNA from tissues and cells / R. Ribaudo, M. Gilman, R.E. Kingston, P. Choczynski, N. Sacchi. - In: CURRENT PROTOCOLS IN IMMUNOLOGY. - ISSN 1934-368X. - 2001:Suppl. 4 CPI(2001 May), pp. 10.11.1-10.11.14. [10.1002/0471142735.im1011s04]
Abstract:
Most procedures for isolating RNA from eukaryotic cells involve lysing and denaturing cells to liberate total nucleic acids. Additional steps are then required to remove DNA.The first basic protocol describes hot phenol extraction of RNA; the method eliminates or minimizes DNA contamination by the shearing of DNA. The second basic protocol allows rapid preparation of total cytoplasmic RNA by using a nonionic detergent to lyse the plasma membrane, leaving the nuclei intact. The nuclei and hence the bulk of the cellular DNA are then removed with a simple brief centrifugation. A guanidinium thiocyanate protocol describes the separation of RNA from other cellular macromolecules in a guanidinium lysate using a CsCl step gradient. A protocol is also provided for isolation of poly(A(+)) mRNAs from total RNA.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
RNA, messenger; animals; humans; cytoplasm; DNA; eukaryotic cells; cell nucleus
Elenco autori:
R. Ribaudo, M. Gilman, R.E. Kingston, P. Choczynski, N. Sacchi
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