Modulation of rat brain protein expression during acute and chronic finasteride administration
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Data di Pubblicazione:
2012
Citazione:
Modulation of rat brain protein expression during acute and chronic finasteride administration / A. Soggiu, M. Bortolato, P. Devoto, C. Neri, C. Piras, A. Urbani, L. Bonizzi, P. Roncada. ((Intervento presentato al convegno EuPA/BSPR proteomics meeting : new horizons and applications for proteomics tenutosi a Glasgow nel 2012.
Abstract:
Introduction: Preliminary data suggest that S5AR inhibitors like finasteride (FlN) may elicit therapeutic
effects in psychotic disorders and Tourette syndrome producing very limited side effects.
Behavioral and neurochemical effects of FIN have been described in rat, but at the moment there are
no proteomic data about effects of FIN administration on rat brain protein expression. In the present
study we evaluated, by gel based and gel free methods, the impact of acute and chronic FIN treatment
on global protein expression in specific rat brain areas.
Methods: Samples were subjected to 2DE. Gels were analyzed using Progenesis SameSpot.
Differentially expressed spots (Mann-Whitney p <0.05) were identified by MALDI-TOF/TOF. Samples
were also analyzed using a shotgun approach (LC-MS). Proteins were classified using PANTHER and
STRING for generation of biological networks
Results: lmage analysis highlighted several significantly up- and down regulated proteins in acute and
chronic group with respect to controls. Shotgun data from nucleus accumbens also showed nine
proteins unique to chronic group and 22 proteins unique to acute group. Classification of proteins and
generation of biological networks are in progress
Conclusion: Present study provides the first evidence for protein variations in rat brain proteome
following FIN treatment. Results indicated several possible candidate proteins modulated by FIN
treatment. Further proteomic and bioinformatics investigations will be necessary to evaluate their role as
possible molecular targets of drug.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
A. Soggiu, M. Bortolato, P. Devoto, C. Neri, C. Piras, A. Urbani, L. Bonizzi, P. Roncada
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