Data di Pubblicazione:
2012
Citazione:
Protein network changes in different time points of human lactation / P. Roncada, A. Soggiu, C. Piras, P. Roggero, A. Budelli, A. Urbani, L. Bonizzi. ((Intervento presentato al convegno EuPA/BSPR proteomics meeting : new horizons and applications for proteomics tenutosi a Glasgow nel 2012.
Abstract:
Introduction: Human milk is a rich source of bioactive proteins that supports the growth and
immunological defences (1) of the newborn. There are several proteomic studies about human milk whey
proteins(2j and fat globule proteins(3) during the lactation period , but at the moment there are no
protein interaction data from all human milk proteins expressed in lactation period. In the present study
we analyzed protein expression and the associated protein network at several time point in lactation
period; i, 7, 15, 30 and 60 days from partum. Eighteen mothers for each time point with fixed criteria of
recruitment were included in study.
Methods: Samples were subjected to 1-DE and 2-DE and all proteins were separated from each
experimental condition were identified by UPLC MS/MS. Biological networks were generated with
STRING.
Results: STRING analysis highlighted several GO-biological processes and GO-molecular functions
mainly involved in defense response to bacteria and enzymatic activities. Classification of proteins and
generation of biological networks were done .
Conclusion: Present study provides the first evidence for protein network modulation in different stages
of human lactation. Results indicated several possible biological processes and molecular functions
modulated in each stage. Proteomic and bioinformatics investigations are necessary to well describe
network role in modulation of nutraceutical and nutritional characteristics of human milk.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
P. Roncada, A. Soggiu, C. Piras, P. Roggero, A. Budelli, A. Urbani, L. Bonizzi
Link alla scheda completa: