Linkage arrangement of RFLP loci in progenies from crosses between doubled haploid Asparagus officinalis L. clones
Articolo
Data di Pubblicazione:
1995
Citazione:
Linkage arrangement of RFLP loci in progenies from crosses between doubled haploid Asparagus officinalis L. clones / F.M. Restivo, F. Tassi, R. Biffi, A. Falavigna, E. Caporali, M.L. Doldi, A. Spada, G. Marziani. - In: THEORETICAL AND APPLIED GENETICS. - ISSN 0040-5752. - 90:1(1995), pp. 124-128.
Abstract:
A preliminary genetic map of the dioecious species Asparagus officinalis L. (2n = 20) has been constructed on the basis of restriction fragment length polymorphism (RFLP) and isozyme marker data. With DNA samples digested with either EcoRI or HindIII 61 out of 148 probes (41%) identified RFLPs in six families of doubled haploid lines obtained through anther culture. A higher level of polymorphism (65%) was observed when a single family was screened for RFLPs using six distinct restriction enzymes. Segregation analysis of the BC progenies (40-80 individuals) resulted in a 418-cM extended map comprising 43 markers: 39 RFLPs, three isozymes and one morphological (sex). These markers are clustered in 12 linkage groups and four of them exhibited significant deviations from the expected 1:1 ratio. One isozyme and three RFLP markers were assigned to the sex chromosome.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Asparagus officinalis L; Genetic map RFLP
Elenco autori:
F.M. Restivo, F. Tassi, R. Biffi, A. Falavigna, E. Caporali, M.L. Doldi, A. Spada, G. Marziani
Link alla scheda completa: