IFN-gamma inhibits c-myc gene expression by impairing the splicing process in a colony-stimulating factor dependent murine myeloid cell line
Articolo
Data di Pubblicazione:
1988
Citazione:
IFN-gamma inhibits c-myc gene expression by impairing the splicing process in a colony-stimulating factor dependent murine myeloid cell line / A. Harel-Bellan, A.T. Brini, W.L. Farrar. - In: JOURNAL OF IMMUNOLOGY. - ISSN 0022-1767. - 141:3(1988 Aug 01), pp. 1012-1017.
Abstract:
Proliferation of NSF-60.8 cells, a CSF-dependent murine myeloid cell line, is strongly inhibited by murine IFN-gamma. Northern analysis of growth arrested, NSF-60.8 cells activated by addition of CSF in the presence or absence of IFN-gamma indicated that IFN-gamma inhibited the induction of c-myc mRNA steady state level by CSF. The effect was observed as early as 30 min after induction and the inhibition was complete after 20 h. IFN-gamma did not impair the transcriptional activation of c-myc gene, and it had only a slight destabilizing effect on the mature c-myc message. Study of the processing steps of c-myc mRNA precursor indicated that in the presence of IFN-gamma, a putative 3.6-kb splice intermediate accumulated instead of the mature message, suggesting that IFN-gamma inhibits the splicing of c-myc precursor.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
A. Harel-Bellan, A.T. Brini, W.L. Farrar
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