Data di Pubblicazione:
1980
Citazione:
ELISA for toxoplasma antibody detection: a comparison with other serodiagnostic tests / A.BALSARI ,G. POLI ,V. MOLINA ,M. DOVIS , E.PETRUZZELLI , A. BONIOLO, E. ROLLERI. - In: AMERICAN JOURNAL OF CLINICAL PATHOLOGY. - ISSN 0002-9173. - 33:7(1980), pp. 640-643.
Abstract:
An ELISA method was developed for the measurement of toxoplasma IgG antibodies in human serum using antigen-coated polystyrene beads as a solid phase and anti human IgG-horse radish peroxidase conjugate as an enzymatic tracer. In order to assess ELISA sensitivity and specificity, a between methods comparison was made using 'conventional' serological tests as reference (dye-test, crossover-linked immunoassay, passive haemagglutination, indirect immunofluorescence). From an analysis of the group classifications obtained some considerations emerged: the ELISA specificity looks comparable with that of the 'reference' tests, as no sample classified as negative by all these tests was ELISA-positive, and vice versa; ELISA appears to correlate better with haemagglutination and immunofluorescence, on the basis of the respective class frequencies; in particular, the number of positives, which is much lower for the dye-test and crossover-linked immunoassay, suggests that a higher sensitivity is reached in the former cases.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
A.BALSARI ,G. POLI ,V. MOLINA ,M. DOVIS , E.PETRUZZELLI , A. BONIOLO, E. ROLLERI
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