Data di Pubblicazione:
1976
Citazione:
The organization of repeated DNA sequences in the human genome / E. Ginelli, G. Corneo. - In: CHROMOSOMA. - ISSN 0009-5915. - 56:1(1976 Jun 30), pp. 55-68.
Abstract:
The arrangement of repetitive and non-repetitive DNA sequences was studied in the human genome. By Ag+-Cs2SO4 density gradient centrifugations of human DNA at different fragment size reannealed to different Cot values and c-RNA hybridization experiments, we have shown the presence of two repetitive DNA fractions, called fast and slow intermediate DNA, with different pattern of sequence organization. The fast intermediate DNA sequences (6% of the genome; CsCl density in renatured form: 1.703 g/ml) are in part clustered in fragments greater than 24,000 nucleotide pairs and in part in fragments ranging from 1,800 to 600 nucleotide pairs spaced with longer more complex sequences. The slow intermediate DNA sequences (30% of the genome; CsCl density in renatured form: 1.707 g/ml) appear to be finely interspersed with non-repetitive sequences. At a DNA fragment size of 600 nucleotide pairs only a third of the slow intermediate DNA sequences are free of unique sequences, while the other two thirds are still organized with unique sequences. It has also been shown that a great amount of the repetitive DNA sequence transcripts in heterogeneous nuclear RNA of HeLa cells are complementary to slow intermediate DNA sequences.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Centrifugation, Density Gradient; Base Sequence; Humans; DNA, Satellite; DNA; Nucleic Acid Renaturation; Nucleic Acid Hybridization
Elenco autori:
E. Ginelli, G. Corneo
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