Expression of bovine leukemia virus ENV glycoprotein in insect cells by recombinant baculovirus
Articolo
Data di Pubblicazione:
1998
Citazione:
Expression of bovine leukemia virus ENV glycoprotein in insect cells by recombinant baculovirus / S. Russo, L. Montermini, T. Berkovitz-Siman, W. Ponti, G. Poli. - In: FEBS LETTERS. - ISSN 0014-5793. - 436:1(1998 Sep), pp. 11-16.
Abstract:
The gp51-p30 glycoprotein constituting BLV envelope was expressed in Sf-21 insect cells by means of recombinant baculoviruses. Post-infection cell lysates mere analyzed, in order to define the immunologic reactivity of recombinant products. Oligosaccharide chains, containing N-acetylglucosamine, mannose, galactose and sialic acid mere found on recombinant gp51-p30. In order to investigate the timing of transcription and translation of the glycoprotein, kinetic assays were carried out on cell lysates and directly in situ on Sf-21 cells during the course of baculovirus infection, The use of different solubilizing reagents mas also evaluated in order to rescue recombinant glycoprotein from its subcellular location.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
baculovirus ; biotechnology ; bovine leukemia virus ; glycosylation ; recombinant glycoprotein
Elenco autori:
S. Russo, L. Montermini, T. Berkovitz-Siman, W. Ponti, G. Poli
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