occurrence of sialidase activity in two distinct and highly homogeneous populations of lysosomes prepared from the brain of developing mouse
Articolo
Data di Pubblicazione:
1991
Citazione:
occurrence of sialidase activity in two distinct and highly homogeneous populations of lysosomes prepared from the brain of developing mouse / A. Fiorilli, C. Siniscalco, A. Chiarini, L. Di Francesco, B. Venerando, G. Tettamanti. - In: FEBS LETTERS. - ISSN 0014-5793. - 282:2(1991 May), pp. 235-238.
Abstract:
Light and heavy lysosomes of mouse forebrain were separated from each other by centrifugation on a Percoll gradient. Light lysosomes were then freed from mitochondria and membranes by sucrose density gradient centrifugation and further purified by floatation-centrifugation on a sucrose gradient. The final preparations of light and heavy lysosomes, fairly homogenous, carried sinlidase activity, assayed on MU-NeuAc. The optimal pH was 4.0 and 4.2, the apparent Km value 2.8 × 10-3 M and 4.2 × 10-3 M and the apparent Vmax value 0.11 and 0.47 mU mg-1 protein, for the light and heavy lysosome sialidase, respectively. From 4 days to adulthood the specific activity of the light and heavy lysosome sialidase increased 3-fold and 1.7-fold, respectively.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Brain development; Lysosome; Sialidase; Subcellular fractionation.
Elenco autori:
A. Fiorilli, C. Siniscalco, A. Chiarini, L. Di Francesco, B. Venerando, G. Tettamanti
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