Data di Pubblicazione:
1993
Citazione:
Molecular organization and chromosomal location of human GC-rich heterochromatic blocks / R. Meneveri, A. Agresti, A. Marozzi, S. Saccone, M. Rocchi, N. Archidiacono, G. Corneo, G. Della Valle, E. Ginelli. - In: GENE. - ISSN 0378-1119. - 123:2(1993 Jan 30), pp. 227-234.
Abstract:
From the sequencing of three genomic DNA fragments and PCR amplification products from total human DNA, we have derived the sequence of a 545-bp Sau3A fragment (68% GC), representative of a family of human DNA repeats. Since previous studies suggested its linkage with unrelated Sau3A repeats of 68 bp (54% GC) (beta-satellite sequences), this feature was further investigated by in situ hybridization experiments and by Southern blot analysis of a panel of DNAs from human-Chinese hamster somatic cell hybrids. Both DNA repeats are preferentially localized on the heterochromatic regions of acrocentric chromosomes, on the pericentromeric heterochromatin of chromosome 1, 3 and 9, and on the proximal euchromatic region of the chromosome Y q arm. On chromosome 9, both repeats are part of a 2.7-kb higher-order repeat unit. These results and the Southern blot analysis on partial digests of total DNA, suggest that the linkage between the two repetitive DNA sequences is a constant feature throughout the genome. Furthermore, Southern blot analysis of HpaII-digested and MspI-digested DNA from different human tissues and tumor cell lines indicates that the investigated heterochromatic blocks appear to be subjected to changes in their methylation pattern.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
human/hamster hybrids; in situ hybridization; methylation; Recombinant DNA; repetitive DNA sequences; β-satellite sequences
Elenco autori:
R. Meneveri, A. Agresti, A. Marozzi, S. Saccone, M. Rocchi, N. Archidiacono, G. Corneo, G. Della Valle, E. Ginelli
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