Studies on analogues of succinic semialdehyde as substrates for succinate semialdehyde dehydrogenase from rat brain
Articolo
Data di Pubblicazione:
1986
Citazione:
Studies on analogues of succinic semialdehyde as substrates for succinate semialdehyde dehydrogenase from rat brain / A.G. Manzocchi, P.A. Biondi, C.L. Secchi, E. Santaniello. - In: JOURNAL OF NEUROCHEMISTRY. - ISSN 0022-3042. - 46:6(1986), pp. 1895-1898.
Abstract:
The methyl ester of succinic semialdehyde (SSA) was examined as a substrate for succinate semialdehyde dehydrogenase (SSADH) from rat brain. It was found that the ester can be oxidized by the enzyme. Values of K(m) for SSA-Me were higher than for those for SSA, and for this substrate the enzyme showed a substrate- dependent inhibition. This finding suggests that the carboxylate group of SSA is not essential in the process of inhibition of SSADH by the substrate. Cyclopropyl analogues of SSA, cis- and trans-1-formyl-cyclopropan-2-carboxylic acids, were also individually tested as substrates of SSADH. Only the trans isomer was found to be oxidized to the corresponding dicarboxylic acid; it inhibited the enzyme in the same range of concentrations as SSA. The above data suggest that, as for γ-aminobutyric acid, SSA is present in an unfolded, transoid conformation at the active site of SSADH.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
A.G. Manzocchi, P.A. Biondi, C.L. Secchi, E. Santaniello
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