Single Strand Conformation Polymorphism (SSCP) for Discrimination of SFB Alleles in Apricot (Prunus armeniaca) S-genotyping
Articolo
Data di Pubblicazione:
2012
Citazione:
Single Strand Conformation Polymorphism (SSCP) for Discrimination of SFB Alleles in Apricot (Prunus armeniaca) S-genotyping / R. Gharesheikhbayat, L. Dondini, D. Bassi, S. Sansavini. - In: ACTA HORTICULTURAE. - ISSN 0567-7572. - 929(2012), pp. 163-167. ((Intervento presentato al 28. convegno IHC – IS on Genomics and Genetic
Transformation of Horticultural Crops.
Abstract:
S-genotyping in self-incompatible fruit tree species is essential to plan
crossing programs and design orchards. Molecular approaches are the best methods
to determine the exact S-allele combination of cultivars either by identifying SRNase
(the female determinant) or SFB (the male determinant). In apricot, PCR
amplification of SFB often produces DNA fragments similar in length; therefore it is
very difficult to identify polymorphisms on agarose gels. To overcome this bottleneck
the SSCP (Single Strand Conformational Polymorphism) technique was applied to
discriminate the allelic variants of this gene and to select the different related
fragments which led to clone and sequence them. Combining the use of PCR and
single-strand conformation polymorphisms (SSCP), seven sequences from the
apricot genome were successfully identified and three new ones were proposed.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
R. Gharesheikhbayat, L. Dondini, D. Bassi, S. Sansavini
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