Data di Pubblicazione:
2012
Citazione:
A qNMR approach for bitterness phenotyping and QTL identification in an F1 apricot progeny / C. Cervellati, C. Paetz, L. Dondini, S. Tartarini, D. Bassi, B. Schneider, A. Masia. - In: JOURNAL OF BIOTECHNOLOGY. - ISSN 0168-1656. - 159:4(2012), pp. 312-319. [10.1016/j.jbiotec.2011.09.004]
Abstract:
In apricot the bitter flavor of seeds is determined by the amount of amygdalin, a cyanogenic glucoside
whose cleavage by endogenous enzymes, upon seed crushing, releases toxic hydrogen cyanide. The presence
of such a poisonous compound is an obstacle to the use and commercialization of apricot seeds for
human or animal nutrition. To investigate the genetic loci involved in the determination of the bitter
phenotype a combined genetic and biochemical approach was used, involving a candidate gene analysis
and a fine phenotyping via quantitative nuclear magnetic resonance, on an F1 apricot progeny. Seven
functional markers were developed and positioned on the genetic maps of the parental lines Lito and
BO81604311 and seven putative QTLs for the bitterness level were determined. In conclusion, this analysis
has revealed some loci involved in the shaping of the bitterness degree; has proven the complexity
of the bitter trait in apricot, reporting an high variance of the QTLs found over the years; has showed
the critical importance of the phenotyping step, whose precision and accuracy is a pre-requisite when
studying such a multifactorial character.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Apricot; Bitterness; Cyanogenic glucosides; Functional marker; Genetic map; NMR; Phenotyping; QTL
Elenco autori:
C. Cervellati, C. Paetz, L. Dondini, S. Tartarini, D. Bassi, B. Schneider, A. Masia
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