Evaluation of a novel serotyping system for hepatitis C virus: strong correlation with standard genotyping methodologies
Articolo
Data di Pubblicazione:
1995
Citazione:
Evaluation of a novel serotyping system for hepatitis C virus: strong correlation with standard genotyping methodologies / V. Dixit, S. Quan, P. Martin, D. Larson, M. Brezina, R. DiNello, K. Sra, Y.N. Lau, D. Chien, J. Kolberg, A. Tagger, G. Davis, A. Polito, G. Gitnick. - In: JOURNAL OF CLINICAL MICROBIOLOGY. - ISSN 0095-1137. - 33:11(1995), pp. 2978-2983.
Abstract:
Direct sequencing and analysis of viral genomes are definitive methods for identifying various hepatitis C virus (HCV) genotypes. However, HCV genome sequencing methods are cumbersome and unsuitable for analyzing large numbers of clinical samples. We have developed a convenient, reliable,and reproducible RIBA strip immunoblot assay system for determining HCV serotype. Briefly, the assay consists of an immunoblot
strip on which there are five lanes of immobilized serotype-specific HCV peptides from the nonstructural
(NS-4) and core regions of the genomes of HCV types 1, 2, and 3. HCV serotype is deduced by determining the greatest intensity of reactivity to the NS-4 serotype-specific HCV peptide band in relation to the intensity of the human immunoglobulin G internal control bands on each strip. HCV core peptide reactivity is used only in the
absence of NS-4 reactivity. We used this assay to successfully serotype a high percentage of sera from well-documented HCV-infected patients. Our serotyping results correlated 99% with the findings from the standard restriction fragment length polymorphism genotyping methods. Less than 5% of the serum samples
were untypeable. For a selected group of alpha interferon-treated patients we observed that the nonresponders (76.2%) and a majority of the responders who relapsed (72.2%) had type 1 HCV infection. A small population
(n 5 8) of complete responders was split 3:4:1 as type 1, type 2, and type 3, respectively. Our data indicate that
this new serotyping assay has the potential to be a highly specific and reliable method for typing of HCV
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
V. Dixit, S. Quan, P. Martin, D. Larson, M. Brezina, R. Dinello, K. Sra, Y.N. Lau, D. Chien, J. Kolberg, A. Tagger, G. Davis, A. Polito, G. Gitnick
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