AUTOMATED FLUOROMETRIC ASSAY PROCEDURE FOR GLUCOHYDROLASES USING A ROUTINE CENTRIFUGAL ANALYZER ASSAY OF ENZYMES OF LYSOSOMAL ORIGIN IN PLASMA .2
Articolo
Data di Pubblicazione:
1995
Citazione:
AUTOMATED FLUOROMETRIC ASSAY PROCEDURE FOR GLUCOHYDROLASES USING A ROUTINE CENTRIFUGAL ANALYZER ASSAY OF ENZYMES OF LYSOSOMAL ORIGIN IN PLASMA .2 / G. GOI, E. GUAGNELLINI, C. BAIRATI, M. BESOZZI, A. LOMBARDO, D. BOLLINI, A. LOVAGNINI, G. TETTAMANTI. - In: EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY. - ISSN 0939-4974. - 33:10(1995), pp. 737-742.
Abstract:
The manual fluorimetric procedure, considered as a reference method for the determination of N-acetyl-beta-D-glucosaminidase, beta-D-glucuronidase and beta-D-galactosidase in human plasma, was automated as a routine method, using the IL Monarch centrifugal analyser. Using a liquid standard with a known enzyme content, the automated assay correlated fairly well with the reference manual method (r values very close to 1). Its analytical imprecision was much lower than that of the manual method. The automated assay of N-acetyl-beta-D-glucosaminidase, beta-D-glucuronidase and beta-D-galactosidase gave coefficients of variation of 5.7-6.9, 3.6-5.0 and 3.8-4.2%, respectively, detection limits of 4, 2 and 1 mU/l plasma respectively, and linear responses of up to 73, 8.4 and 0.9 U/l of plasma respectively. Furthermore, the method required only small volumes of undiluted plasma (4-10 mu l). This method appears to be reliable, sensitive, simple enough for routine analyses and as cost effective as the most common routine serum enzyme assays.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
LYSOSOMAL ENZYMES; BLOOD PLASMA; AUTOMATED FLUORIMETRIC ASSAY; QUALITY CONTROL
Elenco autori:
G. GOI, E. GUAGNELLINI, C. BAIRATI, M. BESOZZI, A. LOMBARDO, D. BOLLINI, A. LOVAGNINI, G. TETTAMANTI
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