Comparative mapping of human alphoid sequences in great apes using fluorescence in situ hybridization
Articolo
Data di Pubblicazione:
1995
Citazione:
Comparative mapping of human alphoid sequences in great apes using fluorescence in situ hybridization / N. Archidiacono, R. Antonacci, R. Marzella, P. Finelli, A. Lonoce, M. Rocchi. - In: GENOMICS. - ISSN 0888-7543. - 25:2(1995), pp. 477-484. [10.1016/0888-7543(95)80048-Q]
Abstract:
Twenty-seven human alphoid DNA probes have been hybridized in situ to metaphase spreads of the common
chimpanzee (PTR), the pigmy chimpanzee (PPA), and the gorilla (GGO) to investigate the evolutionary
relationship between the centromeric regions of the great ape chromosomes. The surprising results showed that the vast majority of the probes did not
recognize their corresponding homologous chromosomes.
Alphoid sequences belonging to the suprachromosomal family 1 (chromosomes 1, 3, 5, 6, 7, 10, 12, 16, and 19) yielded very heterogeneous results: some
probes gave intense signals, but always on nonhomologous chromosomes; others did not produce any hybridization
signal. Almost all probes belonging to the suprachromosomal family 2 (chromosomes 2, 4, 8, 9, 13,
14, 15, 18, 20, 21, and 22) recognized a single chromosome: chromosome 11 (phylogenetic IX) in PTR and PPA and chromosome 19 (phylogenetic V) in GGO. Localization of probes of suprachromosomal family 3 (chromosomes 1, 11, 17, and X) was found to be substantially conserved in PTR and PPA, but not in GGO.
Probe pDMX1, specific for the human X chromosome, was the only sequence detecting its corresponding chromosome in all three species. PPA chromosomes I,
IIp, IIq, IV, V, VI, and XVIII were never labeled, even under low-stringency hybridization conditions, by the 27 alphoid probes used in this study. These results, with particular reference to differences found in the
two related species PTR and PPA, suggest that alphoid centromeric sequences underwent a very rapid evolution.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
N. Archidiacono, R. Antonacci, R. Marzella, P. Finelli, A. Lonoce, M. Rocchi
Link alla scheda completa: