Species-specific DNA probe and development of a quantitative PCR assay for the detection of Morganella morganii
Articolo
Data di Pubblicazione:
2012
Citazione:
Species-specific DNA probe and development of a quantitative PCR assay for the detection of Morganella morganii / C. Ferrario, G. Ricci, F. Borgo, M.G. Fortina. - In: LETTERS IN APPLIED MICROBIOLOGY. - ISSN 0266-8254. - 54:4(2012), pp. 292-298.
Abstract:
Aims: To develop a SYBR Green quantitative PCR assay (qPCR) for the
specific detection of Morganella morganii, a fish pathogen responsible for the
Histamine Fish Poisoning.
Methods and Results: A new primer set, amplifying a 179-bp fragment of the
16S rRNA gene, was selected for specificity, and 14 M. morganii strains and 32
non-Morganella strains were evaluated. The melting temperature of 84 C was
consistently specific for the amplicon. Two standard curves were constructed:
the minimum detection sensitivity was 0Æ563 pg of pure DNA, corresponding
to DNA extracted from nine cells of M. morganii. The qPCR assay was
evaluated in experiments with seeded fish samples, and the regression
coefficient values were calculated.
Conclusions: A highly specific and rapid assay was developed for the detection
of M. morganii in tuna fish samples.
Significance and Impact of the Study: This method represents the first study
about the quantification of pathogenic M. morganii in fish products. This
approach can be utilized to prevent the presence of this undesirable species in
the food chain.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Histamine formers; Morganella morganii; PCR; Quantitative microbial detection; Tuna fish samples
Elenco autori:
C. Ferrario, G. Ricci, F. Borgo, M.G. Fortina
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