Lifespan of encapsulated boar spermatozoa ad different temperatures in sperm controlled release device
Poster
Data di Pubblicazione:
2002
Citazione:
Lifespan of encapsulated boar spermatozoa ad different temperatures in sperm controlled release device / D. Vigo, R. Norberti, M. Faustini, M.L. Torre, S. Stacchezzini, L. Maggi, U. Conte, E. Munari, G. Maffeo. - In: REPRODUCTION IN DOMESTIC ANIMALS. - ISSN 0936-6768. - 37:(2002), p. 256.
Abstract:
Barium alginate controlled release capsules containing semen were
developed to increase the efficiency of the artificial insemination
technique. The stability of encapsulated boar semen was assessed at
different storage temperatures, evaluating non-reacted acrosomes
(NRA) and secondary anomalies (SA). A solution of BaCl2 was added
to semen until a Baþþ
concentration of 5 mM was reached; the
suspension was dropped into a sodium alginate solution. Gel capsules
were obtained, and suspended in an extender for swine semen. Another
kind of capsules was obtained by cross-linking of G caps in a solution of
protamine sulphate. NRA and SA were evaluated in semen soon after
sampling and soon after the encapsulation process. Control semen was
diluted in Zorlesco extender. After 24 hours of incubation at 18 C and 38
C, spermatozoa were stained for NRA and microscopically examined for SA. Semen storage in alginate or protamine alginate capsules enhances the quality of semen in terms of NRA and SA, particularly in
thermal stress conditions (38 C), when cell metabolism is at physiological levels. These advantages could be related to the non-dilution of spermatozoa, simulating the in vivo testicular and epididymal conditions. Twenty-four hours 18 C storage of encapsulated spermatozoa
conserves high NRA percentage and herefore supports the possibility of an in-field employment for instrumental insemination: sperm-life is guaranteed for at least 24 hours.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
D. Vigo, R. Norberti, M. Faustini, M.L. Torre, S. Stacchezzini, L. Maggi, U. Conte, E. Munari, G. Maffeo
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