Data di Pubblicazione:
1993
Citazione:
Oligonucleotide degradation contributes to resistance to antisense compounds / A. Ryte, S. Morelli, M. Mazzei, A. Alama, P. Franco, G. Canti, A.N. Nicolin. - In: ANTI-CANCER DRUGS. - ISSN 0959-4973. - 4:2(1993 Apr), pp. 197-200.
Abstract:
A subline of the human B cell lymphoma DHL-4, grown in the artificial serum-free medium HB101, displayed a resistant phenotype to the activity of an antisense oligodeoxynucleotide (aODN) effective on the parental DHL-4 line. It was found that the cellular uptake of the 18mer aODN in the two cell lines was almost the same. In contrast, the unresponsive subline DHL-4r degraded the aODN very efficiently, in contrast to the stability of aODN inside cells of the parental DHL-4 line. Activation of the degrading 'machinery' combined with selective properties of the artificial medium may be responsible for the loss of responsiveness to aODN.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
antisense oligodeoxynucleotides; cellular uptake; resistant cells; stability
Elenco autori:
A. Ryte, S. Morelli, M. Mazzei, A. Alama, P. Franco, G. Canti, A.N. Nicolin
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