Involvement of cdc25mm/ras-grf1-dependent signaling in the control of neuronal excitability
Articolo
Data di Pubblicazione:
2001
Citazione:
Involvement of cdc25mm/ras-grf1-dependent signaling in the control of neuronal excitability / R. Tonini, S. Franceschetti, D. Parolaro, M. Sala, E. Mancinelli, S. Tininini, R. Brusetti, G. Sancini, R. Brambilla, E. Martegani, E. Sturani, R. Zippel. - In: MOLECULAR AND CELLULAR NEUROSCIENCES. - ISSN 1044-7431. - 18:6(2001), pp. 691-701.
Abstract:
Ras-GRF1 is a neuron-specific guanine nucleotide exchange factor for Ras proteins. Mice lacking Ras-GRF1 (-/-) are severely impaired in amygdala-dependent long-term synaptic plasticity and show higher basal synaptic activity at both amygdala and hippocampal synapses (Brambilla et al.,, 1997). In the present study we investigated the effects of Ras-GRF1 deletion on hippocampal neuronal excitability. Electrophysiological analysis of both primary cultured neurons and adult hippocampal slices indicated that Ras-GRF1-/- mice displayed neuronal hyperexcitability. Ras-GRF1-/- hippocampal neurons showed increased spontaneous activity and depolarized resting membrane potential, together with a higher firing rate in response to injected current. Changes in the intrinsic excitability of Ras-GRF1-/- neurons can entail these phenomena, suggesting that Ras-GRF1 deficiency might alter the balance between ionic conductances. In addition, we showed that mice lacking Ras-GRF1 displayed a higher seizure susceptibility following acute administration of convulsant drugs. Taken together, these results demonstrated a role for Ras-GRF1 in neuronal excitability.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
R. Tonini, S. Franceschetti, D. Parolaro, M. Sala, E. Mancinelli, S. Tininini, R. Brusetti, G. Sancini, R. Brambilla, E. Martegani, E. Sturani, R. Zippel
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