Data di Pubblicazione:
2000
Citazione:
Interaction of human substantia nigra neuromelanin with lipids and peptides / L. Zecca, P. Costi, C. Mecacci, S. Ito, M. Terreni, S. Sonnino. - In: JOURNAL OF NEUROCHEMISTRY. - ISSN 0022-3042. - 74:4(2000), pp. 1758-1765.
Abstract:
Neuromelanin was isolated from human substantia nigra using different procedures. in the pigment isolated by any of these procedures a peptide component covalently bound to the melanic structure was found, as shown by treatment with reagents known to eliminate noncovalently bound proteins. The amino acid content of such a peptide component was reproducible and corresponded to similar to 15% of the neuromelanin weight. Neuromelanin also showed the ability to absorb specifically lipid molecules, similar to 20% of its weight, and among these lipids cholesterol was identified, constituting similar to 5% of the total lipid mixture. A synthetic melanin, incubated with putamen homogenate, bound tissue peptides with an amino acid content quite close to that of neuromelanin. The same synthetic melanin adsorbed a lower amount of lipids from the putamen homogenate compared with neuromelanin. The sulfur content of neuromelanin was also reproducible even using different isolation procedures. A nonpigmented tissue like corpus callosum was used as a control and extracted by the method used for neuromelanin isolation; a total elimination of tissue components was found, thus demonstrating the capability of the reported procedures to isolate neuromelanin alone. The presence of a peptide component in the neuromelanin structure and the selective affinity for lipid molecules suggest new aspects of the functional role and metabolic pathway of neuromelanin.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Neuromelanin; Parkinson's disease; Substantia nigra
Elenco autori:
L. Zecca, P. Costi, C. Mecacci, S. Ito, M. Terreni, S. Sonnino
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