Effect of substrate concentration on the enantioselectivity of cyclohexanone monooxygenase from Acinetobacter calcoaceticus and its rationalization
Articolo
Data di Pubblicazione:
2000
Citazione:
Effect of substrate concentration on the enantioselectivity of cyclohexanone monooxygenase from Acinetobacter calcoaceticus and its rationalization / F. Zambianchi, P. Pasta, G. Ottolina, G. Carrea, S. Colonna, N. Gaggero, J. M. Ward. - In: TETRAHEDRON-ASYMMETRY. - ISSN 0957-4166. - 11:18(2000), pp. 3653-3657. [10.1016/S0957-4166(00)00354-2]
Abstract:
The ee values of lactone 3, and not of lactone 2, obtained from the enantiodivergent oxidation of racemic bicyclo[3.2.0]hept-2-en-6-one 1, catalyzed by cyclohexanone monooxygenase from Acinetobacter calcoaceticus, were found to be markedly dependent on the degree of conversion and substrate concentration. The results are rationalized on the basis of a model which hypothesizes the binding of a second substrate molecule to an enzyme site distinct from the catalytic site.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
F. Zambianchi, P. Pasta, G. Ottolina, G. Carrea, S. Colonna, N. Gaggero, J. M. Ward
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