Data di Pubblicazione:
1992
Citazione:
Intracellular signalling systems controlling the 5 alpha-reductase in glial cell cultures / R. C. Melcangi, F. Celotti, P. Castano, L. Martini. - In: BRAIN RESEARCH. - ISSN 0006-8993. - 585:1-2(1992 Jul 10), pp. 411-415. [10.1016/0006-8993(92)91247-C]
Abstract:
Glial cells are able to metabolize testosterone into DHT through the action of the enzyme 5 alpha-reductase. DHT may be further processed to 3 alpha-diol by the 3 alpha-hydroxysteroid-dehydrogenase. The aim of this study was to analyze if a modulation of two second messenger systems might be able to modify the 5 alpha-reductase and the 3 alpha-hydroxysteroid-dehydrogenase activities present in glial cells. To this purpose, the formation of DHT has been measured in rat glial cell cultures after different time of exposure to TPA, 4 alpha-Ph, an active and an inactive phorbol ester respectively, and 8-Br-cAMP. The results obtained indicate that the formation of DHT is not modified by the addition of phorbol esters. On the contrary, a statistically significant increase of 5 alpha-reductase activity, over control levels, has been observed after 6, 12, and 24 h of incubation with 8-Br-cAMP (10(-3) M). The effect of the cAMP analogue appears to be specific for the 5 alpha-reductase, since the 3 alpha-hydroxysteroid-dehydrogenase did not show any variation after exposure to the drug. In conclusion, the present data suggest that proteinkinase A (PKA) might be involved in the control of the 5 alpha-reductase in glial cells. It is postulated that nervous inputs utilizing cAMP as the second messenger might modify the activity of this enzyme in glial cells.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
5α-Reductase; Cell culture; Glial cell; Rat; Second messenger; Testosterone
Elenco autori:
R.C. Melcangi, F. Celotti, P. Castano, L. Martini
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