Heterogeneous distribution and transmembrane signaling properties of lymphocyte function associated antigen-1 (LFA-1) in human lymphocyte subsets
Articolo
Data di Pubblicazione:
1989
Citazione:
Heterogeneous distribution and transmembrane signaling properties of lymphocyte function associated antigen-1 (LFA-1) in human lymphocyte subsets / R. Pardi, J.R. Bender, C. Dettori, E. Gianazza, E.G. Engleman. - In: JOURNAL OF IMMUNOLOGY. - ISSN 0022-1767. - 143:10(1989), pp. 3157-3166.
Abstract:
The role of LFA-1, a member of the integrin super-gene family, in intercellular adhesion, including lymphocyte-endothelial cell (EC) binding, has been established. We now demonstrate that differences in LFA-1 cell surface density are responsible for the variable adhesion efficiency of lymphocyte subsets to EC. Electrophoretic analysis revealed multiple glycosylated isoforms of both α and β subunits, largely as a result of different degrees of sialization, with variable expression among different lymphocyte subsets. Neuraminidase digestion before EC adhesion increased the binding efficiency of all lymphocyte subsets, although the relative increase in each subset was proportional to the initial LFA-1 sialic acid content. LFA-1 cross-linking resulted in phosphoinositide hydrolysis and a rise in [Ca2+](i) when using anti-α but not anti-β subunit antibodies. These findings indicate that the density of LFA-1 on lymphocyte subsets controls their adhesive properties, and that the LFA-1 α subunit has transmembrane signaling properties that may result in activation events after interaction with its natural ligands.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
R. Pardi, J.R. Bender, C. Dettori, E. Gianazza, E.G. Engleman
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