Identification of spinacine as the principal reaction product of gamma-casein with formaldehyde in cheese
Articolo
Data di Pubblicazione:
1988
Citazione:
Identification of spinacine as the principal reaction product of gamma-casein with formaldehyde in cheese / P. Restani, P. Campagner, A. Fiecchi, P. Resmini, C. L. Galli. - In: FOOD AND CHEMICAL TOXICOLOGY. - ISSN 0278-6915. - 26:5(1988 May), pp. 441-6-446.
Abstract:
Formaldehyde is added to milk in the production of grana cheese as an antimicrobial agent. In order to study the fate of the formaldehyde, a grana cheese preparation was made using 14C-labelled formaldehyde. The 14C-activity in the cheese was found to be mainly associated with the caseins, but it was not uniformly distributed among the different fractions (alpha s, beta- and gamma-caseins). gamma-Casein, separated by electrophoresis, was the most reactive component showing the highest specific activity. In the gamma-casein fractions, 99% of 14C-activity was associated with the basic amino acids. The only radioactive reaction product present in the gamma-casein fraction was identified by HPTLC and by an amino acid analyser to be spinacine, a condensation product of formaldehyde and histidine. Using the same method, other unknown radioactive products, of much less relevance, were detected in the total casein hydrolysate.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Caseins; Formaldehyde; Chromatography, Thin Layer; Chromatography, Ion Exchange; Pyridines; Cheese; Chromatography, High Pressure Liquid; Imidazoles
Elenco autori:
P. Restani, P. Campagner, A. Fiecchi, P. Resmini, C. L. Galli
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