Lipolytic activity of porcine pancreas lipase on fatty acid esters of dialkylglycerols: a structural basis for the design of new substrates for the assay of pancreatic lipases activity
Articolo
Data di Pubblicazione:
2001
Citazione:
Lipolytic activity of porcine pancreas lipase on fatty acid esters of dialkylglycerols: a structural basis for the design of new substrates for the assay of pancreatic lipases activity / P. Ciuffreda, A. Loseto, A. Manzocchi, E. Santaniello. - In: CHEMISTRY AND PHYSICS OF LIPIDS. - ISSN 0009-3084. - 111:2(2001), pp. 105-110.
Abstract:
For the design of new synthetic substrates for the assay of pancreatic lipases activity, acyl dialkylglycerols of variable chain length were prepared. Titrimetric assay of these substrates showed the highest lipolytic activity of porcine pancreas lipase (pPL) with butanoyl dibutylglycerol. The activity is lower but comparable to that shown by pPL towards the classical substrate tributyrin. The 4-nitrophenylcarbonate of 1,2-di-O-butylglycerol, has been prepared and proposed as synthetic substrate for a new spectrophotometric assay of pancreatic lipases.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Dialkylglycerols; Lipolytic activity; Pancreatic lipase; Spectrophotometric assay; Titrimetric assay
Elenco autori:
P. Ciuffreda, A. Loseto, A. Manzocchi, E. Santaniello
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