Spectrophotometric assay of lipase activity: A new 4-nitrophenyl ester of a dialkylglycerol suitable as a chromogenic substrate of Pseudomonas cepacia lipase.
Articolo
Data di Pubblicazione:
2003
Citazione:
Spectrophotometric assay of lipase activity: A new 4-nitrophenyl ester of a dialkylglycerol suitable as a chromogenic substrate of Pseudomonas cepacia lipase. / P. Ciuffreda, S. Casati, A. Loseto, E. Santaniello. - In: BIOCATALYSIS AND BIOTRANSFORMATION. - ISSN 1024-2422. - 21:3(2003), pp. 123-127.
Abstract:
Evaluation of Pseudomonas cepacia lipase (PCL) activity by a titrimetric method with triacylglycerols (TAG) and synthetic dialkylglycerol esters (DAGE) established the chain length selectivity of the enzyme and this information has been used to design a new chromogenic substrate [1,2-di-O-octyl-sn-glycerol-3-O-(4-nitrophenyl) glutarate] for the determination of the lipolytic activity of PCL.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
4-Nitrophenyl esters; Dialkylglycerol esters; Lipase activity; Pseudomonas cepacia lipase; Spectrophotometric assay
Elenco autori:
P. Ciuffreda, S. Casati, A. Loseto, E. Santaniello
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