Bv8, the amphibian homologue of the mammalian prokineticins, induces a pro-inflammatory phenotype of mouse macrophages
Articolo
Data di Pubblicazione:
2006
Citazione:
Bv8, the amphibian homologue of the mammalian prokineticins, induces a pro-inflammatory phenotype of mouse macrophages / C. Martucci , S. Franchi, E. Giannini, H. Tian, P. Melchiorri, L. Negri, P.G. Sacerdote. - In: BRITISH JOURNAL OF PHARMACOLOGY. - ISSN 0007-1188. - 147:2(2006), pp. 225-234.
Abstract:
1 The small protein Bv8, isolated from the amphibian skin, belongs to a novel family of secreted
proteins linked to several biological effects. We describe the expression of Bv8/prokineticins and their
receptors in mouse macrophages, and characterize their proinflammatory activities.
2 The rodent analogue of Bv8, prokineticin-2, is expressed by macrophages, as well as its G-proteincoupled
receptor prokineticin receptor (PKR-1 and PKR-2). PKR-1 is expressed more abundantly.
3 Bv8 induces potent chemotaxis of macrophages at concentrations as low as 10^(-12)M.
4 It stimulates lipopolysaccharide-induced production of the proinflammatory cytokines IL-1 and
IL-12, reducing that of the anti-inflammatory cytokine IL-10. The effects are observed starting at the
very low concentration of 10^(-11)M.
5 Effects on chemotaxis and cytokine are not pertussis-toxin sensitive, but are completely prevented
by addition of the phospholipase inhibitor U73122, suggesting a Gq protein is involved in the Bv8-
induced effects.
6 Studies in PKR-1 knockout mice indicate that all the activities exerted by Bv8 on macrophages are
mediated by the PKR-1 receptor.
7 In conclusion, Bv8 appears to be able to induce the macrophage to migrate and to acquire a
proinflammatory phenotype.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Bv8 ; chemotaxis ; anti-inflammatory cytokines ; macrophages ; prokineticins ; proinflammatory cytokines
Elenco autori:
C. Martucci , S. Franchi, E. Giannini, H. Tian, P. Melchiorri, L. Negri, P.G. Sacerdote
Link alla scheda completa: