Monoclonal antibodies against recombinant human growth hormone as probes to study immune function
Articolo
Data di Pubblicazione:
1996
Citazione:
Monoclonal antibodies against recombinant human growth hormone as probes to study immune function / D. Lattuada, C. Casnici, S. Gregori, A. Berrini, C. Secchi, P. Franco, O. Marelli. - In: HYBRIDOMA. - ISSN 0272-457X. - 15:3(1996 Jun), pp. 211-7-217.
Abstract:
Monoclonal antibodies were raised against human recombinant growth hormone (rhGH) and those that did not cross-react with other human recombinant proteins like prolactin (PRL), interleukin 2 (IL-2), insulin, or bovine pituitary growth hormone were selected. The selected hybridoma supernatants were studied for their ability to influence T lymphocyte proliferation when induced either by a mitogen, such as phytohemagglutinin (PHA), or by alloantigen. All supernatants inhibited proliferation. Three MAbs were then purified by several passages on antimouse IgG (or IgM)- agarose columns, and characterized. These MAbs recognized three different epitopes, as revealed by competition study, although their inhibitory effect on PHA-induced T cell proliferation was quite similar. The data demonstrate that the MAbs were not cytolytic, that they did not interfere with the PHA binding to T cell membranes, and, as revealed by FACS analysis, did not bind to the membrane. Finally, these MAbs immunoprecipitated a 44-kDa molecule from PHA-activated T cell-concentrated supernatants. These data indicate that the MAbs recognized a soluble factor that plays a central role in T cell proliferation and that is probably the immune growth hormone.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Animals; Pituitary Gland; Human Growth Hormone; Humans; Mice; Molecular Weight; Cross Reactions; Insulin; Prolactin; Antibodies, Monoclonal; Cattle; Interleukin-2; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Female; Growth Hormone
Elenco autori:
D. Lattuada, C. Casnici, S. Gregori, A. Berrini, C. Secchi, P. Franco, O. Marelli
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