Idiotypic replica of an anti-human tumor-associated antigen monoclonal antibody. Analysis of monoclonal Ab1 and Ab3 fine specificity
Articolo
Data di Pubblicazione:
1989
Citazione:
Idiotypic replica of an anti-human tumor-associated antigen monoclonal antibody. Analysis of monoclonal Ab1 and Ab3 fine specificity / G Viale, G Flamini, F Grassi, R Buffa, PG Natali, M Pelagi, F Leoni, S Menard, AG Siccardi. - In: JOURNAL OF IMMUNOLOGY. - ISSN 0022-1767. - 143:12(1989), pp. 4338-4344.
Abstract:
CaMBr1 is a tissue-specific and tumor-associated saccharidic epitope, defined by mAb MBr1 (Ab1), expressed on glycoconjugates of the human mammary carcinoma cell line MCF-7 and of normal and neoplastic mammary epithelial cells. An anti-anti-idiotypic monoclonal Ab3, 2G-3, identifying a human breast tumor associated antigen, was raised by using as immunogen a mouse anti-idiotypic monoclonal Ab2, A3B10, which behaves as the internal image of CaMBr1. mAb 2G-3, as well as MBr1, defines a saccharidic epitope on glycoconjugates extracted from MCF-7 cells and shows MBr1-like reactivity on normal and neoplastic-tissues. Experimental evidence, however, suggests that the fine immunoreactivity of the two antibodies is not identical, because MBr1 has a preferential reactivity with glycolipids and 2G-3 with glycoproteins. We suggest that a possible biologic explanation for our findings could reside in the nature of the immunogens used to raise the two mAb (glycolipid vs protein 'internal image').
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
G Viale, G Flamini, F Grassi, R Buffa, PG Natali, M Pelagi, F Leoni, S Menard, AG Siccardi
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