Plasminogen activator inhibitor type-1 synthesis and mRNA expression in HepG2 cells are regulated by VLDL
Articolo
Data di Pubblicazione:
1996
Citazione:
Plasminogen activator inhibitor type-1 synthesis and mRNA expression in HepG2 cells are regulated by VLDL / L. Sironi, L. Mussoni, L. Prati, D. Baldassarre, M. Camera, C. Banfi, E. Tremoli. - In: ARTERIOSCLEROSIS, THROMBOSIS, AND VASCULAR BIOLOGY. - ISSN 1079-5642. - 16:1(1996 Jan), pp. 89-96.
Abstract:
The effect of VLDL on plasminogen activator inhibitor type 1 biosynthesis in HepG2 cells was investigated. Exposure of HepG2 cells to VLDL (range, 10 to 100 micrograms protein per milliliter) for 16 hours resulted in an enhanced release of PAI-1 antigen and PAI activity into conditioned medium, accompanied by the accumulation of intracellular triglycerides. By using a monoclonal antibody (IgG C7) specific to the LDL receptor, we showed that the effect of VLDL is mediated by its interaction with the LDL receptor. Enhanced PAI-1 release was due to increased biosynthesis: PAI-1 mRNA was doubled, mainly because of the effect on the 2.2-kb PAI-1 mRNA rather than the 3.2-kb transcript. Addition of insulin with the VLDL further enhanced PAI-1 antigen release and PAI-1 mRNA accumulation. The effect of VLDL on steady state levels of PAI-1 mRNA was apparently not due to an increase of gene transcription but to stabilization of both PAI-1 mRNA transcripts. The enhancing effect of VLDL on PAI-1 biosynthesis in HepG2 cells may raise PAI-1 antigen levels not only in hypertriglyceridemic states but also in those conditions in which both insulin and VLDL are elevated.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
RNA, Messenger; Liver Neoplasms; Tumor Cells, Cultured; Receptors, LDL; Carcinoma, Hepatocellular; Triglycerides; Humans; Lipoproteins, VLDL; Plasminogen Activator Inhibitor 1; Gene Expression Regulation; Culture Media, Conditioned
Elenco autori:
L. Sironi, L. Mussoni, L. Prati, D. Baldassarre, M. Camera, C. Banfi, E. Tremoli
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