In vivo confocal microscopy of conjunctival roundish bright objects : young, older, and Sjögren subjects
Articolo
Data di Pubblicazione:
2011
Citazione:
In vivo confocal microscopy of conjunctival roundish bright objects : young, older, and Sjögren subjects / E. Villani, S. Beretta, D. Galimberti, F. Viola, R. Ratiglia. - In: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE. - ISSN 0146-0404. - 52:7(2011 Jul), pp. 4829-4832. [10.1167/iovs.10-6215]
Abstract:
PURPOSE. To investigate by laser scanning confocal microscopy
(LSCM) the density of presumed epithelial, presumed goblet,
and presumed inflammatory cells in the tarsal conjunctiva of
healthy young and older subjects and in patients with Sjo¨gren’s
syndrome (SS). To evaluate the interobserver variability and to
compare the measured densities with known age-related and
SS-related changes.
METHODS. The authors studied 24 eyes of 12 healthy young
subjects (8 women, 4 men; average age, 26 years; age range,
21–30 years), 24 eyes of 12 healthy older subjects (10 women,
2 men; average age, 68 years; age range, 67–74 years), and 24
eyes of 12 patients with SS (10 women, 2 men; average age, 62
years; age range, 49–72 years). The inferior tarsal conjunctiva
of each patient was examined in vivo by LSCM. The density of
the three cell types was independently analyzed by two
masked investigators.
RESULTS. The density of presumed epithelial, presumed goblet,
and presumed inflammatory cells was significantly higher in SS
patients than in both control groups (P 0.001; Mann-Whitney
U test). The densities for presumed goblet cells calculated
by the two investigators were significantly different from one
another (P 0.01, Mann-Whitney U test) and were not correlated.
CONCLUSIONS. LSCM is a promising tool that should profoundly
change the study of the ocular surface, but it requires accurate
standardization before it is used in clinical practice.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
E. Villani, S. Beretta, D. Galimberti, F. Viola, R. Ratiglia
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