Data di Pubblicazione:
2009
Citazione:
Tris-lecithin extender supplemented with antioxidant catalase for chilling of canine semen / M. Beccaglia, P. Anastasi, S. Chigioni, G.C. Luvoni. - In: REPRODUCTION IN DOMESTIC ANIMALS. - ISSN 0936-6768. - 44:Suppl. 2(2009 Jul), pp. 345-349.
Abstract:
The aims were to evaluate the suitability of a non-commercial
TRIS-lecithin (LC) extender and the effect of different
concentrations of catalase (CAT) on motility, capacitation
status (Chlortetracycline-assay) and zona pellucida (ZP) binding
capacity of canine spermatozoa stored at +5 C for 4 days.
The sperm-rich fractions of the ejaculates of four stud dogs
were divided into four aliquots. After centrifugation, sperm
pellets were diluted (200 · 106 sperm ⁄ ml) in TRIS buffer, citric
acid, fructose, antibiotics, supplemented with 20% egg yolk
(TRIS-EY) or 0.04% soybean lecithin (TRIS-LC) with CAT
(150 or 450 UI ⁄ ml) or without CAT, and then preserved at
5 C for 4 days. The results showed that LC is a valid
alternative to EY for chilling canine semen, as similar rates
of motility, number of uncapacitated spermatozoa and of
spermatozoa binding the oocyte ZP were obtained in semen
chilled in TRIS-LC or TRIS-EY. Different concentrations of
CAT in a TRIS-LC based extender did not improve the quality
of semen after chilling. However, a concentration of
150 UI ⁄ ml CAT resulted in an increased number of spermatozoa
bound to the oocyte ZP, after 4 days of chilling when
compared to semen chilled with TRIS-EY and TRIS-LC. In
conclusion, an animal protein-free extender with soybean LC,
as a replacement of EY, is suitable for 4 days chilling of canine
spermatozoa, but the addition of CAT does not improve
general semen quality except for a slight effect on sperm-ZP
binding.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Dog ; semen ; chilling ; lecithin
Elenco autori:
M. Beccaglia, P. Anastasi, S. Chigioni, G.C. Luvoni
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