Protein titration curves by combined isoelectric focusing-electrophoresis with hemoglobin mutants as models
Articolo
Data di Pubblicazione:
1978
Citazione:
Protein titration curves by combined isoelectric focusing-electrophoresis with hemoglobin mutants as models / P.G. Righetti, R. Krishnamoorthy, E. Gianazza, D. Labie. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 166:2(1978), pp. 455-460.
Abstract:
By performing electrphoresis perpendicular to a stationary pH gradient generated by focused carrier ampholytes in a gel slab, a pictorial representation of a protein titration curve is obtained. By running a protein and its genetic mutants in parallel, the shape of the relative titration curve reveals which charged amino acid has been substituted. Within a given family of proteins, under a constant set of experimental conditions, the relative mobility at any given pH can be correlated with the number of protons lost or acquired by the protein.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
P.G. Righetti, R. Krishnamoorthy, E. Gianazza, D. Labie
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