Data di Pubblicazione:
2003
Citazione:
Meiosis resumption of canine oocytes cultured in the isolated oviduct / G.C. Luvoni, S. Chigioni, E. Allievi, D. Macis. - In: REPRODUCTION IN DOMESTIC ANIMALS. - ISSN 0936-6768. - 38:5(2003), pp. 410-414.
Abstract:
The aim of this study was to investigate the effects of culture in
isolated oviducts relative to meiotic maturation, the time
required to resume meiosis and the viability of the canine
oocytes. For this purpose, cumulus–oocyte complexes and
isthmus–ampullar tracts of the oviducts were collected from
bitches undergoing ovariohysterectomies and destined to two
experiments of culture. In experiment 1, the oocytes were
cultured for 24 or 30 h: (1) in 100 ll drops under oil; (2) on the
mucosal epithelium of the open oviducts; (3) in the ligated
oviducts. In experiment 2, oocytes were cultured in the ligated
oviduct for 24, 30 and 48 h. A group of control oocytes was
not cultured (0 h). The results showed that within 30 h of
culture, a higher proportion of oocytes (p < 0.001) resumed
meiosis in the ligated oviduct (63.8%) than in drop (20.4%) or
in the open oviduct (27.1%). Moreover, 24 and 30 h of culture
assured higher proportions of meiosis resumption than 48 h
(69.2and 59.1% vs 35.8%, p < 0.005). Oocyte resumption of
meiosis was mainly determined by oocytes at meiotic stages
preceding metaphase I, while stages between metaphase I and
II in the ligated oviduct ranged between 12.5 and 31.9%. The
extension of the culture time up to 48 h in the oviduct
increased oocyte degeneration significantly (59.3%, p <
0.0001) compared with 24 and 30 h (18.7 and 27.3%,
respectively) and the oviductal epithelium showed nuclear
picnosis and degeneration following culture. The present study
suggests that the close physical interaction between the canine
oocytes and the oviductal tract positively affects oocyte
maturation, and meiosis is resumed within 30 h of culture.
Moreover, the oocyte survival is better preserved within 30 h
in the ligated oviduct compared with the conventional culture
in drop or to the culture in the open oviduct, but the ligated
oviduct does not assure viability of the oocytes up to 48 h of culture.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Dog ; oocyte ; nuclear maturation
Elenco autori:
G.C. Luvoni, S. Chigioni, E. Allievi, D. Macis
Link alla scheda completa: