Isolation and sequence analysis of the gene encoding triose phosphate isomerase from Zygosaccharomyces bailii
Articolo
Data di Pubblicazione:
2001
Citazione:
Isolation and sequence analysis of the gene encoding triose phosphate isomerase from Zygosaccharomyces bailii / A. Merico, F. Rodrigues, M. Côrte-Real, D. Porro, B. M. Ranzi, C. Compagno. - In: YEAST. - ISSN 0749-503X. - 18:9(2001), pp. 775-780.
Abstract:
The ZbTPI1 gene encoding triose phosphate isomerase (TIM) was cloned from a Zygosaccharomyces bailii genomic library by complementation of the Saccharomyces cerevisiae tpi1 mutant strain. The nucleotide sequence of a 1.5 kb fragment showed an open reading frame (ORF) of 746 bp, encoding a protein of 248 amino acid residues. The deduced amino acid sequence shares a high degree of homology with TIMs from other yeast species, including some highly conserved regions. The analysis of the promoter sequence of the ZbTPI1 revealed the presence of putative motifs known to have regulatory functions in S. cerevisiae.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
glycolysis ; triose phosphate isomerase ; spoilage yeast ; Zygosaccharomyces bailii
Elenco autori:
A. Merico, F. Rodrigues, M. Côrte-Real, D. Porro, B. M. Ranzi, C. Compagno
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