Data di Pubblicazione:
1991
Citazione:
PROPERTIES OF A SYNAPTIC VESICLE PROTEIN-BINDING PLASMA-MEMBRANES / M. POPOLI, R. PATERNO. - In: NEUROREPORT. - ISSN 0959-4965. - 2:2(1991), pp. 93-95.
Abstract:
SYNAPTIC vesicles contain a protein (agglutinin) which binds cell plasma membranes. The protein activity is titrated measuring the agglutination of rabbit trypsinized-fixed red blood cells induced by serial dilutions of purified vesicles. The protein is highly concentrated in vesicles: the specific activity is 150-fold higher in vesicles compared to the brain homogenate, and 20-fold higher compared to the crude synaptosomal membrane fraction. The binding is specifically inhibited by the four major brain sialoglycosphingolipids, with a higher affinity for polysialo- rather than for monosialoganglioside (GM1) IC50 are 3.9 x 10(-5) M (GT1b), 8.9 x 10(-5) M (GD1a), 2.8 x 10(-4) M (GD1b) and 4.9 x 10(-4) M (GM1). Cells which are not agglutinated by the vesicles (human red blood cells) can be activated by incubation with gangliosides: insertion of the glycolipids in the plasma membranes makes them sensitive to the vesicle haemagglutinin.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
SYNAPTIC VESICLES ; MEMBRANE PROTEIN ; SYNAPTIC TRANSMISSION ; GANGLIOSIDES
Elenco autori:
M. POPOLI, R. PATERNO
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