Data di Pubblicazione:
1992
Citazione:
ISOLATED P65 PROTEIN REPRODUCED MEMBRANE-BINDING ACTIVITY OF SYNAPTIC VESICLES / M. POPOLI, R. PATERNO. - In: NEUROREPORT. - ISSN 0959-4965. - 3:2(1992), pp. 177-180.
Abstract:
Purified synaptic vesicles are highly enriched with a protein which binds cell plasma membranes. The binding is selective for acidic phospholipids and sialoglycosphingolipids. In partition chromatography of vesicle proteins, the binding activity was co-eluted with a limited set of proteins. Among them the most abundant species were two vesicle-specific proteins: p65 and synaptophysin. In affinity chromatography of vesicle proteins, only p65 bound to a column of immobilized lysoganglioside. The same protein, purified by preparative electrophoresis, retained the binding activity and fully reproduced the hemagglutinating property and the selectivity for acidic lipids of whole vesicles. The results suggest that the (hemagglutinating) lipid binding properties of the vesicles are mainly if not exclusively due to p65.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
SYNAPTIC VESICLES ; MEMBRANE PROTEIN ; SYNAPTIC TRANSMISSION ; PHOSPHOLIPIDS
Elenco autori:
M. POPOLI, R. PATERNO
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