A general method for fractionation of plasma proteins. Dye-ligand affinity chromatography on immobilized Cibacron blue F3-GA
Articolo
Data di Pubblicazione:
1982
Citazione:
A general method for fractionation of plasma proteins. Dye-ligand affinity chromatography on immobilized Cibacron blue F3-GA / E. Gianazza, P. Arnaud. - In: BIOCHEMICAL JOURNAL. MOLECULAR ASPECTS. - ISSN 0306-3275. - 201:1(1982 Jan 01), pp. 129-136. [10.1042/bj2010129]
Abstract:
The chromatographic behaviour of 27 different plasma proteins on fractionation of human plasma on immobilized Cibacron Blue F3-GA was studied. The column was eluted by using a three-step procedure. First, a low-molarity buffer (30 mM-H3PO4/Na3PO4, pH 7.0, I0.053) was used, then a linear salt gradient (0-1 M-NaCl in the buffer above) was applied, followed by a wash with two bed volumes of 1.0 M-NaCl. Finally, bound proteins were 'stripped' with 0.5 M-NaSCN. Up to 1 ml of whole plasma could be loaded per 5 ml bed volume. No denaturation of proteinase inhibitors or complement fractions was observed. The recovery of individual proteins ranged between 52 and greater than 95%. Enrichment of four individual plasma components (alpha 1-antitrypsin, caeruloplasmin, antithrombin III and haemopexin) was between 10-fold and 75-fold. These results indicate that chromatography on immobilized Cibacron Blue F3-GA can be a useful initial step in the purification of plasma proteins.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Chromatography, Affinity ; Coloring Agents ; Blood Proteins ; Immunoelectrophoresis ; Electrophoresis, Polyacrylamide Gel ; Humans ; Anthracenes ; Triazines ; Blood Protein Electrophoresis
Elenco autori:
E. Gianazza, P. Arnaud
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