Data di Pubblicazione:
1980
Citazione:
Human globin chain separation by isoelectric focusing in ultrathin polyacrylamide gels / K. Valkonen, E. Gianazza, P.G. Righetti. - In: CLINICA CHIMICA ACTA. - ISSN 0009-8981. - 107:3(1980), pp. 223-229.
Abstract:
We describe three basic modifications of our previous method for thalassemia screening by isoelectric focusing of heme-free globin chains: the gel thickness is reduced from 2 mm to 240 μm; the level of the detergent Nonidet P-40 in the gel is decreased from 3% to 0.5%; the polyacrylamide slab is covalently fixed to the supporting glass plate by treatment with silane A-174. By the present method the entire focusing process, starting from gel moulding up to gel destaining and drying, is completed within 4 h, a fraction of the time needed in our previous technique. More than a 100 samples can be analyzed per working day. The present technique also affords increased sensitivity: less than 1μg protein/band is detected and less than 200 picomol of hemoglobin are needed for each analysis. Band sharpness and resolution in our ultrathin gels is also considerably increased.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
K. Valkonen, E. Gianazza, P.G. Righetti
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