Data di Pubblicazione:
1990
Citazione:
2-DIMENSIONAL MAPS IN THE MOST EXTENDED (PH 2.5-11) IMMOBILIZED PH GRADIENT INTERVAL / P. SINHA, M. PRAUS, E. KOTTGEN, E. GIANAZZA, P. RIGHETTI. - In: JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS. - ISSN 0165-022X. - 21:2(1990), pp. 173-179.
Abstract:
In conventional isoelectric focusing in soluble buffers, it has been quite difficult to produce two-dimensional (2-D) separation sin pH intervals greater than pH 4-8. In general more alkaline proteins wer analyzed by non-equilibrium IEF in the first dimension. Even with the advent of immobilized pH gradients (IPG), separations could be extended to pH gradients not wider than pH 3-10, due to a lack of suitable buffers. Since more acidic and more alkaline acrylamido buffers have recently been synthesized, we have been able to optimize what is beleived to be the widest possible immobilized pH gradient, a h 2.5-11 span. We report here for the first time 2-D separations of total tissue lysates in such extended pH 2.5-11 gradients. It appears that, with the IPG technique, close to 100% of all possible cell products can be displayed in a single 2-D map.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Immobilized pH gradient; Isoelectric focusing; Two-dimensional map
Elenco autori:
P. SINHA, M. PRAUS, E. KOTTGEN, E. GIANAZZA, P. RIGHETTI
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