Basic proteins and basic membranes adjusting blotting and staining conditions to immobilon CD
Articolo
Data di Pubblicazione:
1995
Citazione:
Basic proteins and basic membranes adjusting blotting and staining conditions to immobilon CD / E. Gianazza, P. Coari, M. Lovati, C. Manzoni, E. Ghibaudi, M. Salmona. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 698:1-2(1995), pp. 351-359.
Abstract:
Transfer efficiency from polyacrylamide gels and binding to Immobilon P and CD were tested in different buffers with 125I-labelled proteins. With a derivatized poly(vinylidene difluoride) membrane, a pH 8 medium was found to be superior to a more alkaline solution, for both acidic and basicproteins. New staining protocols were tried on ImmobilonCD. Toluidine Blue and iodine vapour gave a negative and a positive stain, respectively, with a fair band-to-background contrast. Protein sequencing after both stains was not impaired by interfering peaks. Biuret solution stained the protein bands pale pink but, even after copper removal with a chelating agent, it completely prevented Edman degradation. The first two procedures compare favourably with a commercial kit for protein detection, Quick Stain, that provides comparable sensitivity but results in several spurious peaks on protein sequencing.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
E. Gianazza, P. Coari, M. Lovati, C. Manzoni, E. Ghibaudi, M. Salmona
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