PURIFICATION OF HUMAN-HEMOGLOBIN VALENCE INTERMEDIATES BY PREPARATIVE IMMOBILIZED PH GRADIENTS
Articolo
Data di Pubblicazione:
1987
Citazione:
PURIFICATION OF HUMAN-HEMOGLOBIN VALENCE INTERMEDIATES BY PREPARATIVE IMMOBILIZED PH GRADIENTS / C. GELFI, P. RIGHETTI, E. ROVIDA, M. SAMAJA. - In: JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS. - ISSN 0165-022X. - 14:3(1987), pp. 139-147.
Abstract:
We compare three separation techniques for preparative purposes, i.e. ion-exchange chromatography on CM-cellulose, conventional isoelectric focusing in polyacrylamide gel slabs and immobilized pH gradients. The biological system used to test the three methods is a solution containing four hemoglobin (Hb) valence intermediates, i.e. metHb, oxyHb, (α+βO2)2 and (αO2β+)2. The ΔpI between the two valence intermediates is 0.04 pH units. Immobilized pH gradients give the best performance in terms of resolving power, total amount of protein which can be loaded and retention of biological activity by the protein (the latter assessed by determination of CO dissociation rates).
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Hemoglobin valence intermediates; Immobilized pH gradients; Isoelectric focusing; Preparative electrophoresis
Elenco autori:
C. GELFI, P. RIGHETTI, E. ROVIDA, M. SAMAJA
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